The Charm® Peel Plate® HET Microbial Test (Peel Plate HET) is a simplified culture
method for detecting micro-organisms.
Each plate contains prepared
media in a shallow dish with an adhesive top. Quickly pipette the test sample
in the middle of the plate and it automatically wicks through the media and
solidifies. No spreading device is needed. Invert the test and stack up to
20 together for efficient incubation. The Peel Plate HET is based on R2A
agar and will produce red colonies for easy visual identification. An air gap
between the plate and cover allows colony picking for additional isolation and
identification.
Benefits of the Peel Plate HET Test Include
Ready-to-use. Just add sample/dilution. No spreading device needed.
Etched grid lines for estimated counts.
Colonies easily picked for additional isolation/classification.
Convenient plate stacking for improved air circulation.
Buffered formulation – pH not required for most samples.
12 month refrigerated shelf-life in a resealable foil zip bag.
47 mm diameter convenient for water filters and 100 mL water samples or larger.
Automated counting and data storage when used with the Charm Peel Plate
Colony Counter
FOR DETECTION AND ENUMERATION OF LIVE
HETEROTROPHIC BACTERIA IN WATER
The Peel Plate HET test is based on R2A agar from Standard Methods for the Examination of
Water and Wastewater, Section 9215. The HET media contains the redox-indicator substrate
2,3,5-triphenyltetrazolium chloride (TTC). TTC is reduced by most heterotrophic bacteria to
produce red colored colonies. The media also contains gelling and wicking agents which absorb
and self-wick.
The Peel Plate HET test is applicable to drinking water, process water, treated water, and 0.45
μm filtered drinking and process water. Samples should be 10-fold serially diluted into the
countable range of 30 to 300 CFU/mL for the spread plate method and 20-200 CFU for the
membrane filter method.
