The Charm® Peel Plate® EC Microbial Test (Peel Plate EC)
is a simplified culture method for detecting micro-organisms.

Each plate contains prepared media in a shallow dish with an adhesive top. Quickly pipette the test sample in the middle of the plate and it automatically wicks through the media and solidifies. No spreading device is needed. Invert the test and stack up to 20 together for efficient incubation. The Peel Plate EC test is based on VRB media. Coliforms will produce red colonies, and E. coli will produce blue/purple colonies for easy visual identification. An air gap between the plate and cover allows colony picking for additional isolation and identification.

Benefits of the Peel Plate CC Test Include

• Ready-to-use. Just add sample/dilution. No spreading device needed.
• Etched grid lines for estimated counts.
• Colonies easily picked for additional isolation/classification.
• Convenient plate stacking for improved air circulation.
• Buffered formulation – pH not required for most samples.
• 12 month refrigerated shelf-life in a resealable foil zip bag.
• Robust performance with neutralizing buffers used in environmental surface samples.
• 47 mm diameter convenient for water filters and 100 mL water samples or larger.
• AOAC-RI and NCIMS Validated Matrices.
• 5 mL high volume test available.
• Automated counting and data storage when used with the Charm Peel Plate Colony Counter

FOR DETECTION AND ENUMERATION OF TOTAL COLIFORM BACTERIA INCLUDING E.COLI

Peel Plate EC medium is based on EC broth medium to support and colorimetrically differentiate the growth of coliform and E. coli in test samples at 35 ± 1 °C. Peel Plate EC tests contains the enzyme substrates salmon-gal (6-chloro-3-indolyl-B-D-galactopyranoside) used to detect ß-galactosidase produced by coliform and x-glucuronide (5-bromo-4-chloro-3-indolyl-B-D-glucuronide) used to detect ß-glucuronidase produced by E. coli when incubated at 35±1 °C. Peel Plate EC tests also contains gelling and wicking agents which absorb and diffuse the sample.

Peel Plate EC test has been certified by the AOAC Research Institute as Performance Tested Method #061501.

This test kit’s performance was reviewed by AOAC-RI and was found to perform to the manufacturer’s specifications. The method has been validated for detection of total coliform in liquid dairy (raw and pasteurized milk, skim, chocolate, cream), solid dairy (reconstituted non-fat dry milk, lactose reduced, vanilla ice cream, sour cream, condensed/evaporated, other flavored, condensed whey), and cultured dairy homogenized in sodium bisulfite diluent before testing (cottage cheese, yogurt, shredded cheese) and found not significantly different from reference method Violet Red Bile Agar (VRBA) with colonies confirmed with Brilliant Green Lactose Bile (BGLB) broth. At 35±1 °C Peel Plate EC test has been validated to distinguish coliform from E. coli in ground meat, environmental surface sponges, carcass rinse and sponges, and 0.45 μm filtered drinking and vegetable/fruit process water, and found not significantly different from FDA-BAM or USDA-FSIS or EPA official reference methods. Samples should be 10-fold serially diluted into the countable range of 1 to 154 CFU/mL.

Download Product Sheet

Peel Plate® EC Microbial Test Brochure