Charm Peel Plate EB Microbial Test for Dairy, Food Dilutions, and Water Samples
The Charm® Peel Plate® EB Microbial Test (Peel Plate EB) is a simplified culture
method for detecting EnterobacteriaceaeE bacteria.
Each plate contains prepared
media in a shallow dish with an adhesive top. Quickly pipette the test sample in the
middle of the plate and it automatically wicks through the media and solidifies. No
spreading device is needed. Invert the test and stack up to 20 together for efficient
incubation. The Peel Plate EB test uses conventional EB formulation with 24-48 hr
incubation depending on the matrix. An air gap between the plate and cover allows
colony picking for additional isolation and identification. A sodium bisulfite diluent is
required for testing cultured dairy products and can be ordered with test kits.
Benefits of the Peel Plate CC Test Include
Ready-to-use. Just add sample/dilution. No spreading device needed.
Etched grid lines for estimated counts.
Colonies easily picked for additional isolation/classification.
Convenient plate stacking for improved air circulation.
Buffered formulation – pH not required for most samples.
12 month refrigerated shelf-life in a resealable foil zip bag.
Robust performance with neutralizing buffers used in environmental surface
samples.
47 mm diameter convenient for water filters and 100 mL water samples or larger.
Works with the USDA Neutralizing Peptone Buffer for carcass rinse.
Automated counting and data storage when used with the Charm Peel Plate
Colony Counter
FOR DETECTION AND ENUMERATION OF ENTEROBACTERIACEAE BACTERIA
The Peel Plate EB test is based on bile salt selective agar, glucose, and multiple colorimetric
enzyme substrates to support growth and colorimetrically identify the growth of the family of
enterobacteriaceae bacteria. The media also contains gelling and wicking agents which absorb
and diffuse the sample.
Peel Plate Enterobacteriaceae AOAC Official Methods of Analysis Announcement AOAC OMA 2018.05
The Peel Plate EB test is applicable to multiple food matrices incubated in the dark at 37 °C
± 1 °C for 24 to 48 hours. The method has been validated according to ISO 16140-2 (2016)
for certification by MicroVal for two food categories: 1-Heat processed milk and dairy products,
and 2-infant formula and infant cereals. Test portions preparation was conducted in accordance
with ISO 6887 parts 1, 4 and 5. The method has also been validated for AOAC Performance
Tested MethodSM (PTM) certification by the AOAC Research Institute for Heat processed milk and
dairy products, infant formula and infant cereals and environmental surface samples (sponge
swab of stainless steel, chicken carcass rinse, and hog hide sponge samples). The Peel Plate EB
method has been compared to a reference method, ISO 21528 parts 1 and 2 methods, and no
significant differences were observed during the validations. The Peel Plate EB method may also
be applicable to other dairy products, meats, eggs, processed foods, pet foods and their contact
surfaces but have not been officially validated by certification organizations.
Samples should be 10-fold serially diluted into the countable range of 1 to 150 CFU/mL.
