Charm® Peel Plate® CC Microbial Test (Peel Plate CC)

Each plate contains prepared media in a shallow dish with an adhesive top. Quickly pipette the test sample in the middle of the plate and it automatically wicks through the media and solidifies. No spreading device is needed. Invert the test and stack up to 20 together for efficient incubation. The Peel Plate CC test is based on VRB media. Coliforms will produce red colonies for easy visual identification. An air gap between the plate and cover allows colony picking for additional isolation and identification. A sodium bisulfite diluent is required for testing cultured dairy products and can be ordered with test kits.

Benefits of the Peel Plate CC Test Include

• Ready-to-use. Just add sample/dilution. No spreading device needed.
• Etched grid lines for estimated counts.
• Colonies easily picked for additional isolation/classification.
• Convenient plate stacking for improved air circulation.
• Buffered formulation – pH not required for most samples.
• 12 month refrigerated shelf-life in a resealable foil zip bag.
• Robust performance with neutralizing buffers used in environmental surface samples.
• 47 mm diameter convenient for water filters and 100 mL water samples or larger.
• 5 mL high volume test available.
• Automated counting and data storage when used with the Charm Peel Plate Colony Counter

FOR DETECTION AND ENUMERATION OF TOTAL COLIFORM BACTERIA

Peel Plate CC medium is based on EC broth medium to support and colorimetrically differentiate the growth of coliform in test samples at 35 ± 1 oC. Peel Plate CC tests contains the enzyme substrate salmon-gal (6-chloro-3-indolyl-B-D-galactopyranoside) used to detect ß-galactosidase produced by coliform. Peel Plate CC tests also contains gelling and wicking agents which absorb and diffuse the sample.

Peel Plate CC test is very similar to the Peel Plate EC test except that the enzyme indicator for generic E. coli, X-gluc, has been removed. Peel Plate EC has been certified by the AOAC Research Institute as Performance Tested Method #061501. This test kit’s performance was reviewed by AOAC-RI and was found to perform to the manufacturer’s specifications. The method has been validated for detection of total coliform in liquid dairy (raw and pasteurized milk, skim, chocolate, cream), solid dairy (reconstituted non-fat dry milk, lactose reduced, vanilla ice cream, sour cream, condensed/ evaporated, other flavored, condensed whey), and cultured dairy homogenized in sodium bisulfite diluent before testing (cottage cheese, yogurt) and found not significantly different from reference method Violet Red Bile Agar (VRBA) with colonies confirmed with Brilliant Green Lactose Bile (BGLB) broth. Some cheddar and other hard cheeses like Parmesan, Romano, Swiss, Provolone use a separately formulated method, Peel Plate CC-C. At 35 ± 1 oC Peel Plate EC test has been validated in ground meat, environmental surface sponges, carcass rinse and sponges, and 0.45 μm filtered drinking and vegetable/fruit process water, and found not significantly different from FDA-BAM or USDA-FSIS or EPA official reference methods. Samples should be 10-fold serially diluted into the countable range of 1 to 154 CFU/mL.

Download Product Sheet

Peel Plate® CC Microbial Test Brochure