Quantitation of Coliforms and E. coli, and Indication/Absence of Hydrogen Sulfide Producing Enterobacteriaceae in Foods

PathoGel is a single assay that selectively grows, detects and quantitates coliform, E.coli and hydrogen sulfide producing Enterobacteriaceae in food and water. This single test can be used for both finished product and process stage testing.

PATHOGEL TEST AT A GLANCE

• 3 Bacterial Groups with 1 Test
• Presence/Absence/Quantity
• Single Use, One Step, No Preparation

Simple One PathoGel tests one sample. No preparation, no wasted reagents, no sample manipulation.

Bio-safe A self enclosed bactericide is burst into the completed test without opening the bag. A 7-8 log reduction of cultured bacteria is carried out in the closed environment with no need to autoclave.

HACCP Friendly PathoGel’s sensitivity and CFU numeration provide a test to verify that food and water contain less than specified indicator levels of organisms. PathoGel establishes baseline levels that demonstrate control of hazard and continual process improvement, all in a user friendly format.

Quantitation of Coliforms and E. coli, and Indication/Absence of Hydrogen Sulfide Producing Enterobacteriaceae in Foods

PathoGel uses a combination of special nutrients, inorganic salts, and detergents to support coliform, E. coli, and Salmonella growth while suppressing the growth of non-coliforms. Specific inducers and chromogenic substrate (X-GAL) form distinctive blue/green color spots around coliform (beta-galactosidase producing bacteria). Fluorogenic substrate (MUG) forms fluorescent spots (under UV light) around E. coli (beta-galactosidase and beta-glucuronidase producing bacteria). Soluble Fe⁺⁺ ions are precipitated by hydrogen sulfides, produced by Salmonella and other hydrogen sulfide producing Enterobacteriaceae, forming black precipitate. A gel-polymerizing agent restricts substrate diffusion and bacterial motility.

The PathoGel Test is a simple procedure. PathoGel can simultaneously detect and identify a single colony forming unit (CFU) of coliform and E. coli or indicate the presence/absence of hydrogen sulfide producing Enterobacteriaceae in a 100 ml water sample or food dilution sample. The distinctive blue/green color formation allows testing in brownish, turbid, and heterotrophic bacterial contaminated waters.

Juice, milk, and meat rinse water may be tested with PathoGel.

100 ml of water or rinse water solution may be tested directly. These sample volumes, depending on targeted detection levels, may be added to 99 ml sterile Butterfield’s Buffer:

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PathoGel Test Brochure