Charm® KIS Test for the Meat Industry

The Charm® KIS Test (Kidney Inhibition Swab) is a simple inhibition test for screening broad-spectrum antimicrobial drugs in feeds, water, urine, serum and tissue

The Charm® KIS Test (Kidney Inhibition Swab) is a simple inhibition test for screening broad-spectrum antimicrobial drugs in fresh or frozen/thawed kidney tissue. KIS reagents are self-contained, solvent-free, and pre-measured in a single-use disposable swab format. Results are obtained in approximately 3 hours. Speed, sensitivity, and simplicity give KIS clear advantages over conventional tests used in the meat industry, such as FAST, SOS and STOP tests.

Charm KIS Test is the USDA (US Department of Agriculture) FSIS (Food Safety and Inspection Service) screening test for antimicrobial drugs in bovine, porcine, ovine, caprine and poultry kidney and muscle tissue under the National Residue Program (FSIS notice 50-09). As of December 2012, the KIS Test is the in-plant drug residue screening test in all federally inspected slaughter establishments and for all State MPI (Meat and Poutry Inspection) programsA.

^AUSDA, FSIS, CLG-ADD 3.02

Simple Procedure

Charm KIS Test:

• Detects antimicrobial drugs at or near Kidney Tolerances and MRLs
• Saves time and labor - Results in approximately 3 hours
• Functions for HACCP and residue avoidance programs
• Works for feeds, water, urine, serum and tissue testing
• Works in laboratory, slaughter house, or on-farm

The Charm KIS (Kidney Inhibition Swab) Test is a microbial inhibition test for antimicrobial drugs.

Bacteria, cultured in agar with purple pH indicator media and kidney swab extract, generate acid that produces a yellow color. In the presence of antibiotic, the bacterial growth is inhibited and the test remains blue/purple.

Sensitivity and Selectivity

Concentration of antimicrobial drugs that cause positive results compared to Kidney Tolerances and MRLs

• ^A Antimicrobial drugs listed are representative of their respective drug families. Test may detect other members of a drug family. Different analogs will have different sensitivities.
• ^B Sensitivity determined by testing unfrozen pork and beef tissue samples (diaphragms) from freshly slaughtered animal and fortifying the swab with antibiotic based on absorbed sample weight. Cautions interpreted as negative.
• ^C Parts per billion or μg/kg.
• ^DMaximum Residue Limit
• ^E Sensitivity to tulathromycin is significantly less than the US Tolerance and EU MRL, however other MRLs range from 1000 to 9000 ppb. Drug specific confirmation of positive test samples should be performed to avoid condemnation of samples containing less than the tolerance or MRL levels
• ^F Contact us for other matrices; feed, water, urine, serum, and tissue

Interferences and Cross Reactivity

There are no known interferences from the following animal drugs at 1000 ppb: chlorothiazide, dexamethasone, furosemide, ivermectin, novobiocin, oxytocin, phenylbutazone, and trichlormethiazide. Other antimicrobial drugs may be detected.

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KIS Test brochure