Fast Alkaline Phosphatase Test (F-AP) Results in 45 Seconds†
PASTEURIZATION VERIFICATION
Alkaline Phosphatase is a natural milk enzyme that is
destroyed by conventional pasteurization (161° F / 72°C
for 15 seconds). This makes Alkaline Phosphatase an
excellent marker to verify destruction of pathogenic
microorganisms in HTST pasteurized dairy products.
Charm® F-AP test is the fastest available phosphatase
method to verify completeness of pasteurization.
The simple one-step procedure requires no reagent
preparation* and delivers results in just 45 seconds†.
With a limit of detection of 20 mU/L, well below
established US and EU regulatory thresholds of 350
mU/L, F-AP provides an early warning indicator of
improper pasteurization.
PMO AND NCIMS APPROVED
As of October 2009, the F-AP test is an official approved
pasteurization test for milk and dairy products by the US
National Conference of Interstate Milk Shippers (NCIMS)
under the Pasteurized Milk Ordinance (PMO). F-AP is
based on the popular NCIMS-approved Charm PasLite
test, an ISO/IDF standard method for testing alkaline
phosphatase in milk and dairy products (ISO 22160/IDF
209). Using a simplified procedure, F-AP provides test
results in just 45 seconds†.
† Creams and chocolate milks require 90 second analysis
* Chocolate milk requires centrifugation
SIMPLE PROCEDURE
PASLITE AT A GLANCE
One Step Test
No Reagents or Incubation Required
Auto Calibration (no manual calculations needed
Results in mU/L
Limit of Detection: 20 mU/L
Phosphatase is a natural milk enzyme that is destroyed by conventional pasteurization. Charm F-AP Fast
Alkaline Phosphatase Test measures phosphatase in dairy products. F-AP Assay calibrated novaLUM
channels provide results in mU/L of phosphatase activity. Levels of 350 mU/L or higher exceed public
health guidelines for pasteurized dairy drinks.
HHST (Higher Heat Shorter Time: >191°F/89°C for 1 second), or aseptically pasteurized dairy products
that are room temperature stable, may contain reactivated phosphatase and, therefore, should not be
tested using this procedure.
MULTIPLE APPLICATIONS
The F-AP test can be used in applications
including:
Routine monitoring of HTST samples
After breaking a seal
Verify HTST sysem or “Vat” pasteurization
Verify safety of heat-treated waste milk fed
to calves to reduce risk of disease transfer